Methods: NIH/3T3 cell line

Samples: NIH/3T3 cells

Species: Mus Musculus

Cell Type: Embryonic fibrobalst

Techniques: RNA-seq

Data processing for CellPalmSeq: The reference transcriptome was acquired from The RNAseq reads were downloaded from the Gene Expression Omnibus ( For each sample, RNAseq expression values (TPM) at the gene level were obtained using kallisto (Bray et al., 2016) with the appropriate reference transcriptome by summing the TPM values of individual isoforms.

Heatmap units: Transcripts per million (TPM)

GEOSample IDTreatmentCell_sourcePMIDYear
Dataset 1: GSE66286GSM1618725; GSM1618726; GSM1618727NoneATCC (CRL-1658)264462582015
Dataset 2: GSE92775GSM2437210; GSM2437211; GSM2437212DMSO treatment, 8 hoursATCC295815852018
Dataset 3: GSE94595GSM2479024NoneEuropean Collection of Animal Cell Cultures (ECACC) (Salisbury, UK)287753822017
Dataset 4: GSE98674GSM2609311NoneATCC (CRL-1658)288863852017
Dataset 5: GSE142732GSM4238342; GSM4238343PBS for 72hNo source provided, but did ensure authentication: NIH/3T3 cells were authenticated using the STR (short tandem repeat) test (Boster, Wuhan, China)325410912020