Methods: CHO cell line
Samples: CHO cells
Species: Cricetulus griseus
Cell Line Origin: Chinese hamster ovary epithelial cell
Techniques: RNA-seq
Data processing for CellPalmSeq: The reference transcriptome was acquired from https://ensembl.org. The RNAseq reads were downloaded from the Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/). For each sample, RNAseq expression values (TPM) at the gene level were obtained using kallisto (Bray et al., 2016) with the appropriate reference transcriptome by summing the TPM values of individual isoforms.
Heatmap units: Transcripts per million (TPM)
| GEO | Sample ID | Treatment | Cell_source | PMID | Year | Strain |
|---|---|---|---|---|---|---|
| Dataset 1: GSE77800 | GSM2059626; GSM2059627; GSM2059628; GSM2059629 | None | Life Technologies (A11557-01) | 27883890 | 2016 | CHO-S |
| Dataset 2: GSE157729 | GSM4774048; GSM4774049 | Empty vector transfection control | Gibco (A29127) | 35301123 | 2022 | ExpiCHO-S |
| Dataset 3: GSE100908 | GSM2696081; GSM2696082; GSM2696083 | None | Sigma (CHOGS-1VL) | 29316330 | 2018 | CHOZN |
| Dataset 4: GSE199531 | GSM5974752; GSM5974753; GSM5974754; GSM5974755 | None | ATCC (CCL-61) | 35625909 | 2022 | CHO-K1 |
| Dataset 5: GSE75521 | GSM1958459; GSM1958460 | None | ATCC (CCL-61) | 28074024 | 2016 | CHO-K1 |