Methods: Calu-3 cell line

Samples: Calu-3 cells

Species: Homo sapiens

Cell Line Origin: Non-small-cell lung carcinoma

Techniques: RNA-seq

Data processing for CellPalmSeq: The reference transcriptome was acquired from The RNAseq reads were downloaded from the Gene Expression Omnibus ( For each sample, RNAseq expression values (TPM) at the gene level were obtained using kallisto (Bray et al., 2016) with the appropriate reference transcriptome by summing the TPM values of individual isoforms.

Heatmap units: Transcripts per million (TPM)

GEOSample IDTreatmentCell_sourcePMIDYear
Dataset 1: GSE125259GSM3567369; GSM3567370; GSM356737124h incubation with empty vehicleATCC (HTB-55)319842102020
Dataset 2: GSE151513GSM4579950; GSM4579951; GSM4579952NoneATCC (HTB-55)339377242021
Dataset 3: GSE162323GSM4949718; GSM4949719NoneATCC (HTB-55)339798332021
Dataset 4: GSE166209GSM5065891; GSM5065892; GSM5065893; GSM5065894DMSO for 12hATCC (HTB-55)340101422021